Aspiration cytology, also known as Fine Needle Aspiration Cytology (FNAC), is a diagnostic procedure in which a thin, hollow needle is inserted into a mass, lump, or organ to extract cells for microscopic examination.
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Purpose:
To diagnose lumps or swellings (especially in the thyroid, breast, lymph nodes, etc.).
To differentiate between benign (non-cancerous) and malignant (cancerous) lesions.
To detect inflammatory, infectious, or cystic conditions.
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Procedure:
1. The area to be examined is cleaned and sometimes numbed.
2. A fine needle (usually 22–25 gauge) attached to a syringe is inserted into the lesion.
3. Negative pressure is applied to aspirate cells into the syringe.
4. The aspirated material is then smeared onto glass slides, stained, and examined under a microscope.
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Advantages:
Quick and simple procedure.
Minimally invasive (no major surgery).
Low cost and few complications.
Gives rapid diagnosis for clinical management.
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Limitations:
Cannot always give definitive diagnosis (especially if sample is inadequate).
Does not show tissue architecture (unlike biopsy).
May need confirmation by histopathology in doubtful cases.
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Common Sites for FNAC:
Thyroid gland
Breast lumps
Lymph nodes
Liver and lung lesions
Salivary glands
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Common Stains Used:
May–Grünwald–Giemsa (MGG) stain
Papanicolaou (Pap) stain
Hematoxylin and Eosin (H&E) stain
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Interpretation:
Cytological findings are categorized as:
Benign
Malignant
Atypical/Suspicious
Non-diagnostic/Inadequate sample
Benign (in Aspiration Cytology)
Meaning:
A benign lesion is non-cancerous. The cells do not invade nearby tissues or spread to other parts of the body.
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Cytology Features of Benign Lesions:
Cells look normal or only slightly abnormal.
Uniform size and shape of nuclei (no pleomorphism).
Well-differentiated (cells resemble normal tissue).
No necrosis or mitotic activity.
Clean background in the smear.
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Examples of Benign Conditions Diagnosed by Aspiration Cytology:
Thyroid: Colloid goiter
Breast: Fibroadenoma
Lymph node: Reactive hyperplasia
Salivary gland: Pleomorphic adenoma
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